Elaboration of a Phytomedicine for Intestinal Comfort Based on the European Union Regulation on Traditional Use: Mixture Design Optimization

Nowadays, knowledge about the uses of medicinal plants is growing exponentially, and in order to conserve valorize this knowledge, European Union has set up multiple regulations control their usage by population. In framework, work based on EU elaborate a new phytomedicine for intestinal comfort. The subject elaboration under category Traditional Use Herbal Medicine Products (TUHMPs), containing approved use at least 30 years, including 15 years (Myrtus communis L. leaves (MC), Pimpinella anisum seeds (PA) Carum carvi (CC) fruit). pharmacopeia monographs recognize individual effect each plant selected, aims study combined using mixture design. Acetic acid-induced writhing test mice used efficacy as pain relief According result, individual, binary ternary combination were very effective compared negative positive approving that synergy between plants. Following design analysis, best formulation was with different ratios (MC: 45%, PA: 52% CC 3%). predicted studied after result. This provided practical way establish phytomedicines within could be model outcoming market.